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Comparative proteomics analysis of high n-butanol producing metabolically engineered Clostridium tyrobutyricum.
Ma C, Kojima K, Xu N, Mobley J, Zhou L, Yang ST, Liu XM. Ma C, et al. J Biotechnol. 2015 Jan 10;193:108-19. doi: 10.1016/j.jbiotec.2014.10.036. Epub 2014 Nov 5. J Biotechnol. 2015. PMID: 25449011
We performed a comparative proteomics analysis, covering 78.1% of open reading frames and 95% of core enzymes, using wild type, ACKKO mutant (deltaack) producing 37.30 g/L of butyrate and ACKKO-adhE2 mutant (deltaack-adhE2) producing 16.68 g/L of butanol. In ACKKO- …
We performed a comparative proteomics analysis, covering 78.1% of open reading frames and 95% of core enzymes, using wild type, ACKKO mutant …
Extending carbon chain length of 1-butanol pathway for 1-hexanol synthesis from glucose by engineered Escherichia coli.
Dekishima Y, Lan EI, Shen CR, Cho KM, Liao JC. Dekishima Y, et al. J Am Chem Soc. 2011 Aug 3;133(30):11399-401. doi: 10.1021/ja203814d. Epub 2011 Jul 7. J Am Chem Soc. 2011. PMID: 21707101
The C4-acyl-CoA intermediates were first synthesized via acetyl-CoA acetyltransferase (AtoB), 3-hydroxybutyryl-CoA dehydrogenase (Hbd), crotonase (Crt), and trans-enoyl-CoA reductase (Ter) from various organisms. The butyryl-CoA synthesized was further extended to hexanoyl …
The C4-acyl-CoA intermediates were first synthesized via acetyl-CoA acetyltransferase (AtoB), 3-hydroxybutyryl-CoA dehydrogenase (Hbd), crot …
[Cloning and expression of key genes of butanol synthetic pathway in Escherichia coli].
Zhang Y, Zhou P, Wang P, Xie J, Ye Q. Zhang Y, et al. Wei Sheng Wu Xue Bao. 2012 May 4;52(5):588-93. Wei Sheng Wu Xue Bao. 2012. PMID: 22803344 Chinese.
We constructed the recombinant strain E. coli pBAT (BCS operon-adhE2-thil/pTrc99a/MG1655). We used 0.1 mmol/l Isopropyl beta-D-thiogalactopyranoside (IPTG) to induce the recombinant E. coli pBAT for 5 h for recombinant protein expression. ...RESULTS: In the recombinant E. …
We constructed the recombinant strain E. coli pBAT (BCS operon-adhE2-thil/pTrc99a/MG1655). We used 0.1 mmol/l Isopropyl beta-D-thioga …
Engineering coenzyme A-dependent pathway from Clostridium saccharobutylicum in Escherichia coli for butanol production.
Ye W, Li J, Han R, Xu G, Dong J, Ni Y. Ye W, et al. Bioresour Technol. 2017 Jul;235:140-148. doi: 10.1016/j.biortech.2017.03.085. Epub 2017 Mar 18. Bioresour Technol. 2017. PMID: 28365341
In this study, recombinant Escherichia coli harboring heterologous coenzyme A-dependent pathway from C. saccharobutylicum DSM 13864 was constructed, which consisted of solventogenic pathway genes: acetoacetyl-CoA thiolase (thlA), aldehyde/alcohol dehydrogenase (adhE2) and …
In this study, recombinant Escherichia coli harboring heterologous coenzyme A-dependent pathway from C. saccharobutylicum DSM 13864 was cons …